the seven-thousandth of an inch in diameter. Leeuwenhoek saw that this muddy stuff was in reality a liquid, in which there were floating this immense number of definitely shaped particles, all aggregated in heaps and lumps and some of them separate. That discovery remained, so to speak, dormant for fully a century, and then the question was taken up by a French discoverer, who, paying great attention and having the advantage of better instruments than Leeuwenhoek had, watched these things and made the astounding discovery that they were bodies which were constantly being reproduced and growing; than when one of these rounded bodies was once formed and had grown to its full size, it immediately began to give off a little bud from one side, and then that bud grew out until it had attained the full size of the first, and that, in this way, the yeast particle was undergoing a process of multiplication by budding, just as effectual and just as complete as the process of multiplication of a plant by budding; and thus this Frenchman, Cagniard de la Tour, arrived at the conclusion--very creditable to his sagacity, and which has been confirmed by every observation and reasoning since--that this apparently muddy refuse was neither more nor less than a mass of plants, of minute living plants, growing and multiplying in the sugary fluid in which the yeast is formed. And from that time forth we have known this substance which forms the scum and the lees as the yeast plant; and it has received a scientific name--which I may use without thinking of it, and which I will therefore give you--namely, "Torula." Well, this was a capital discovery. The next thing to do was to make out how this torula was related to the other plants. I won't weary you with the whole course of investigation, but I may sum up its results, and they are these--that the torula is a particular kind of a fungus, a particular state rather, of a fungus or mould. There are many moulds which under certain conditions give rise to this torula condition, to a substance which is not distinguishable from yeast, and which has the same properties as yeast--that is to say, which is able to decompose sugar in the curious way that we shall consider by-and-by. So that the yeast plant is a plant belonging to a group of the Fungi, multiplying and growing and living in this very remarkable manner in the sugary fluid which is, so to speak, the nidus or home of the yeast.
That, in a few words, is, as far as investigation--by the help of one's eye and by the help of the microscope--has taken us. But now there is an observer whose methods of observation are more refined than those of men who use their eye, even though it be aided by the microscope; a man who sees indirectly further than we can see directly--that is, the chemist; and the chemist took up this question, and his discovery was not less remarkable than that of the microscopist. The chemist discovered that the yeast plant being composed of a sort of bag, like a bladder, inside which is a peculiar soft, semifluid material--the chemist found that this outer bladder has the same composition as the substance of wood, that material which is called "cellulose," and which consists of the elements carbon and hydrogen and oxygen, without any nitrogen. But then he also found (the first person to discover it was an Italian chemist, named
Fabroni, in the end of the last century) that this inner matter which was contained in the bag, which constitutes the yeast plant, was a substance containing the elements carbon and hydrogen and oxygen and nitrogen; that it was what Fabroni called a vegeto-animal substance, and that it had the peculiarities of what are commonly called "animal products."
This again was an exceedingly remarkable discovery. It lay neglected for a time, until it was subsequently taken up by the great chemists of modern times, and they, with their delicate methods of analysis, have finally decided that, in all essential respects, the substance which forms the chief part of the contents of the yeast plant is identical with the material which forms the chief part of our own muscles, which forms the chief part of our own blood, which forms the chief part of the white of the egg; that, in fact, although this little organism is a plant, and nothing but a plant, yet that its active living contents contain a substance which is called "protein," which is of the same nature as the substance which forms the foundation of every animal organism whatever.
Now we come next to the question of the analysis of the products, of that which is produced during the
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