feed upon complex foods instead of the simple ones, which form the food of green plants. We may have no hesitation, then, in calling them plants. It is interesting to notice that with this idea their place in the organic world is reduced to a small one systematically. They do not form a class by themselves, but are simply a subclass, or even a family, and a family closely related to several other common plants. But the absence of chlorophyll and the resulting peculiar life has brought about a curious anomaly. Whereas their closest allies are known only to botanists, and are of no interest outside of their systematic relations, the bacteria are familiar to every one, and are demanding the life attention of hundreds of investigators. It is their absence of chlorophyll and their consequent dependence upon complex foods which has produced this anomaly.
CLASSIFICATION OF BACTERIA.
While it has generally been recognised that bacteria are plants, any further classification has proved a matter of great difficulty, and bacteriologists find it extremely difficult to devise means of distinguishing species. Their extreme simplicity makes it no easy matter to find points by which any species can be recognised. But in spite of their similarity, there is no doubt that many different species exist. Bacteria which appear to be almost identical, under the microscope prove to have entirely different properties, and must therefore be regarded as distinct species. But how to distinguish them has been a puzzle. Microscopists have come to look upon the differences in shape, multiplication, and formation of spores as furnishing data sufficient to enable them to divide the bacteria into genera. The genus Bacillus, for instance, is the name given to all rod-shaped bacteria which form endogenous spores, etc. But to distinguish smaller subdivisions it has been found necessary to fall back upon other characters, such as the shape of the colony produced in solid gelatine, the power to produce disease, or to oxidize nitrites, etc. Thus at present the different species are distinguished rather by their physiological than their morphological characters. This is an unsatisfactory basis of classification, and has produced much confusion in the attempts to classify bacteria. The problem of determining the species of bacteria is to-day a very difficult one, and with our best methods is still unsatisfactorily solved. A few species of marked character are well known, and their powers of action so well understood that they can be readily recognised; but of the great host of bacteria studied, the large majority have been so slightly experimented upon that their characters are not known, and it is impossible, therefore, to distinguish many of them apart. We find that each bacteriologist working in any special line commonly keeps a list of the bacteria which he finds, with such data in regard to them as he has collected. Such a list is of value to him, but commonly of little value to other bacteriologists from the insufficiency of the data. Thus it happens that a large part of the different species of bacteria described in literature to- day have been found and studied by one investigator alone. By him they have been described and perhaps named. Quite likely the same species may have been found by two or three other bacteriologists, but owing to the difficulty of comparing results and the incompleteness of the descriptions the identity of the species is not discovered, and they are probably described again under different names. The same process may be repeated over and over again, until the same species of bacterium will come to be known by several different names, as it has been studied by different observers.
VARIATION OF BACTERIA.
This matter is made even more confusing by the fact that any species of bacterium may show more or less variation. At one time in the history of bacteriology, a period lasting for many years, it was the prevalent opinion that there was no constancy among bacteria, but that the same species might assume almost any of the various forms and shapes, and possess various properties. Bacteria were regarded by some as stages in the life history of higher plants. This question as to whether bacteria remain constant in character for any considerable length of time has ever been a prominent one with bacteriologists, and even to-day we hardly know what the final answer will be. It has been demonstrated beyond peradventure that some species may change their physiological characters. Disease bacteria, for instance, under certain conditions lose their powers of developing disease. Species which sour milk, or others which turn gelatine green, may lose their characters. Now, since it is upon just such physiological characters as these that we must depend in order to separate different species of bacteria from each other, it will be seen that great confusion
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